Visualization of chorioretinal vasculature in mice in vivo using a combined OCT/SLO imaging system

Mayank Goswami; Pengfei Zhang; Edward N. Pugh Jr.; Robert J. Zawadzki

doi:10.1117/12.2213609

2 May 2016 Visualization of chorioretinal vasculature in mice in vivo using a combined OCT/SLO imaging system

Mayank Goswami, Pengfei Zhang, Edward N. Pugh Jr., Robert J. Zawadzki

Proceedings Volume 9693, Ophthalmic Technologies XXVI; 96930G (2016) https://doi.org/10.1117/12.2213609
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Chorioretinal blood vessel morphology in mice is of great interest to researchers studying eye disease mechanisms in animal models. Two leading retinal imaging modalities -- Optical Coherence Tomography (OCT) and Scanning Laser Ophthalmoscopy (SLO) -- have offered much insight into vascular morphology and blood flow. OCT “flow-contrast” methods have provided detailed mapping of vascular morphology with micrometer depth resolution, while OCT Doppler methods have enabled the measurement of local flow velocities. SLO remains indispensable in studying blood leakage, microaneurysms, and the clearance time of contrast agents of different sizes. In this manuscript we present results obtained with a custom OCT/SLO system applied to visualize the chorioretinal vascular morphology of pigmented C57Bl/6J and albino nude (Nu/Nu) mice. Blood perfusion maps of choroidal vessels and choricapillaris created by OCT and SLO are presented, along with detailed evaluation of different OCT imaging parameters, including the use of the scattering contrast agent Intralipid. Future applications are discussed.

Conference Presentation

Citation Download Citation

Mayank Goswami, Pengfei Zhang, Edward N. Pugh Jr., and Robert J. Zawadzki "Visualization of chorioretinal vasculature in mice in vivo using a combined OCT/SLO imaging system", Proc. SPIE 9693, Ophthalmic Technologies XXVI, 96930G (2 May 2016); https://doi.org/10.1117/12.2213609

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