4 March 2016 A comparison study of Riboflavin/UV-A and Rose-Bengal/Green light cross-linking of the rabbit corneas using optical coherence elastography
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Proceedings Volume 9693, Ophthalmic Technologies XXVI; 96931T (2016) https://doi.org/10.1117/12.2213395
Event: SPIE BiOS, 2016, San Francisco, California, United States
The biomechanical properties of the cornea are critical factors which determine its health and subsequent visual acuity. Keratoconus is a structural degeneration of the cornea which can diminish vision quality. Riboflavin/UV-A corneal collagen cross-linking (UV-CXL) is an emerging treatment that increases the stiffness of the cornea and improves its ability to resist further degeneration. While UV-CXL has shown great promise for effective therapy of the keratoconus, there are concerns associated with the UV irradiation, such as keratocyte cytotoxicity. Rose-bengal/green light corneal collagen cross-linking (RGX) has been proposed as an alternative to UV-CXL. Because of the high absorbance of the rose-bengal dye at green wavelengths, the treatment time is significantly shorter than with UV-CXL. Moreover, because green light is used in lieu of UV irradiation, there are no cytotoxic side-effects. In this study, noncontact optical coherence elastography (OCE) was used to compare the outcomes of UV-CXL and RGX treatment in rabbit cornea. Low-amplitude (micrometer scale) elastic waves were induced by a focused air-pulse loading system. The elastic wave propagation was then imaged by a phase-stabilized swept source OCE (PhS-SSOCE) system. The changes in the viscoelasticity of the corneas were quantified by a previously developed modified Rayleigh Lamb frequency model. The depth-resolved micro-scale phase-velocity distribution in the cornea was used to reveal the depth-wise heterogeneity before and after both cross-linking techniques. Our results show that UV-CXL and RGX increased the stiffness of the corneas by ~54% and ~5% while reducing the viscosity by ~42% and ~17%, respectively. The depth-wise phase velocities showed that UV-CXL affected the anterior ~1/3 of the corneas, while RGX only affected the anterior ~1/7 of the corneas.
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Jiasong Li, Jiasong Li, Manmohan Singh, Manmohan Singh, Zhaolong Han, Zhaolong Han, Srilatha Vantipalli, Srilatha Vantipalli, Chih-Hao Liu, Chih-Hao Liu, Chen Wu, Chen Wu, Raksha Raghunathan, Raksha Raghunathan, Tina Kazemi, Tina Kazemi, Michael D. Twa, Michael D. Twa, Kirill V. Larin, Kirill V. Larin, "A comparison study of Riboflavin/UV-A and Rose-Bengal/Green light cross-linking of the rabbit corneas using optical coherence elastography", Proc. SPIE 9693, Ophthalmic Technologies XXVI, 96931T (4 March 2016); doi: 10.1117/12.2213395; https://doi.org/10.1117/12.2213395

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