Efficiency of photodynamic therapy using indocyanine green and infrared light on MCF-7 breast cancer cells in vitro

Mustafa Kemal Ruhi; Ayşe Ak; Murat Gülsoy

doi:10.1117/12.2214308

1 March 2016 Efficiency of photodynamic therapy using indocyanine green and infrared light on MCF-7 breast cancer cells in vitro

Mustafa Kemal Ruhi, Ayşe Ak, Murat Gülsoy

Author Affiliations +

Proceedings Volume 9694, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXV; 96940Q (2016) https://doi.org/10.1117/12.2214308
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Cancer is one of the main reasons of death in all around the world. The main treatments of cancer include surgical intervention, radiation therapy and chemotherapy. These treatments can be applied separately or in a combined manner. Another therapeutic method that is still being researched and recently has started to be used in clinical applications is Photodynamic Therapy (PDT). Most photosensitizers currently being investigated are sensitive to red light. However, it is known that infrared light has a better penetration into the skin or tissue. Indocyanine Green (ICG), which is used in this study, is sensitive to infrared light. The aim of this in vitro study is to investigate the effect of PDT on breast cancer cells by using different doses of ICG and infrared light irradiation. 25, 50 and 100 μM ICG concentrations and 25 and 50 J/cm² laser energy doses were applied to MCF-7 cell lines. MTT analyses were performed on 24, 48 and 72 hours following the treatments. As a result, inhibition of cell viability was observed in a time and dose dependent manner. It can be concluded that ICG-PDT application is a good alternative to conventional radiation therapy and chemotherapy for breast cancer treatment.

Conference Presentation

Citation Download Citation

Mustafa Kemal Ruhi, Ayşe Ak, and Murat Gülsoy "Efficiency of photodynamic therapy using indocyanine green and infrared light on MCF-7 breast cancer cells in vitro", Proc. SPIE 9694, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXV, 96940Q (1 March 2016); https://doi.org/10.1117/12.2214308

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