1 March 2016 Photodynamic inactivation of contaminated blood with Staphylococcus aureus
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Abstract
The presence of bacteria in the bloodstream can trigger a serious systemic inflammation and lead to sepsis that cause septic shock and death. Studies have shown an increase in the incidence of sepsis over the years and it is mainly due to the increased resistance of microorganisms to antibiotics, since these drugs are still sold and used improperly. The bacterial contamination of blood is also a risk to blood transfusions. Thus, bacteria inactivation in blood is being studied in order to increase the security of the blood supply. The purpose of this study was to decontaminate the blood using the photodynamic inactivation (PDI). Human blood samples in the presence of Photogem® were illuminated at an intensity of 30 mW/cm2, and light doses of 10 and 15 J/cm2. Blood counts were carried out for the quantitative evaluation and blood smears were prepared for qualitative and morphological evaluation by microscopy. The results showed normal viability values for the blood cells analyzed. The light doses showed minimal morphological changes in the membrane of red blood cells, but the irradiation in the presence of the photosensitizer caused hemolysis in red blood cells at the higher concentrations of the photosensitizer. Experiments with Staphylococcus aureus, one of the responsible of sepsis, showed 7 logs10 of photodynamic inactivation with 50 μg/mL and 15 J/cm2 and 1 log10 of this microorganism in a co-culture with blood.
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Thaila Quatrini Corrêa, Thaila Quatrini Corrêa, Natalia Mayumi Inada, Natalia Mayumi Inada, Sebastião Pratavieira, Sebastião Pratavieira, Kate Cristina Blanco, Kate Cristina Blanco, Cristina Kurachi, Cristina Kurachi, Vanderlei Salvador Bagnato, Vanderlei Salvador Bagnato, "Photodynamic inactivation of contaminated blood with Staphylococcus aureus", Proc. SPIE 9694, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXV, 969414 (1 March 2016); doi: 10.1117/12.2213701; https://doi.org/10.1117/12.2213701
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