8 March 2016 Photodynamic antimicrobial chemotherapy (PACT) against oral microorganisms with the use of blue LED associated to curcumin
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Abstract
The use of curcumin as antimicrobial agent has been suggested and this effect may be potentialized by appropriate light. This study evaluated the effect of PACT using blue LED (λ450ηm ± 5ηm, 220mW and spot of 0.785 cm2) associated to Curcumin at different concentrations (75, 37.5, 18.75, 9.37 and 4.68 μg /mL). Microorganisms from the oral mucosa and the posterior region of the tongue were collected and inoculated into test tubes containing 8mL of TSB medium. For these assays were performed 16 readings. In the assays were used culture plate of 24 wells. To each well was added 400 μL of the suspension containing the microorganisms. Suspensions without curcumin were placed in eight wells. Elsewhere, curcumin was applied varying concentrations with pre-irradiation time of 5 min. After stirring, 200 μL aliquot was taken from each well and the readings were immediately carried out by a spectrophotometer (SPECTRA MAX). Assessments of turbidity were performed following CLSI standard methods. After 1 hour of incubation in a bacteriological oven, 200 μL aliquot was removed from the remaining wells for a second reading. The results showed a decrease of total microorganisms in the most of test groups. The best result of the PACT was with 75 μg/mL, showing 81% of inhibition. It is concluded that PACT with blue LED associated to Curcumin could be a potential mechanism for controlling microorganism proliferation on the oral cavity.
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Fernando José Pires Sampaio, Fernando José Pires Sampaio, Gustavo M. Pires-Santos, Gustavo M. Pires-Santos, Susana C. P. S. de Oliveira, Susana C. P. S. de Oliveira, Juliana S. C. Monteiro, Juliana S. C. Monteiro, Vanderlei S. Bagnato, Vanderlei S. Bagnato, Antônio L. B. Pinheiro, Antônio L. B. Pinheiro, "Photodynamic antimicrobial chemotherapy (PACT) against oral microorganisms with the use of blue LED associated to curcumin", Proc. SPIE 9695, Mechanisms of Photobiomodulation Therapy XI, 96950O (8 March 2016); doi: 10.1117/12.2213515; https://doi.org/10.1117/12.2213515
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