Fluorescence guided lymph node biopsy in large animals using direct image projection device

Elizabeth Ringhausen; Tylon Wang; Jonathan Pitts; Walter J. Akers

doi:10.1117/12.2209657

4 March 2016 Fluorescence guided lymph node biopsy in large animals using direct image projection device

Elizabeth Ringhausen, Tylon Wang, Jonathan Pitts, Walter J. Akers

Proceedings Volume 9696, Molecular-Guided Surgery: Molecules, Devices, and Applications II; 96960K (2016) https://doi.org/10.1117/12.2209657
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The use of fluorescence imaging for aiding oncologic surgery is a fast growing field in biomedical imaging, revolutionizing open and minimally invasive surgery practices. We have designed, constructed, and tested a system for fluorescence image acquisition and direct display on the surgical field for fluorescence guided surgery. The system uses a near-infrared sensitive CMOS camera for image acquisition, a near-infra LED light source for excitation, and DLP digital projector for projection of fluorescence image data onto the operating field in real time. Instrument control was implemented in Matlab for image capture, processing of acquired data and alignment of image parameters with the projected pattern. Accuracy of alignment was evaluated statistically to demonstrate sensitivity to small objects and alignment throughout the imaging field. After verification of accurate alignment, feasibility for clinical application was demonstrated in large animal models of sentinel lymph node biopsy. Indocyanine green was injected subcutaneously in Yorkshire pigs at various locations to model sentinel lymph node biopsy in gynecologic cancers, head and neck cancer, and melanoma. Fluorescence was detected by the camera system during operations and projected onto the imaging field, accurately identifying tissues containing the fluorescent tracer at up to 15 frames per second. Fluorescence information was projected as binary green regions after thresholding and denoising raw intensity data. Promising results with this initial clinical scale prototype provided encouraging results for the feasibility of optical projection of acquired luminescence during open oncologic surgeries.

Conference Presentation

Citation Download Citation

Elizabeth Ringhausen, Tylon Wang, Jonathan Pitts, and Walter J. Akers "Fluorescence guided lymph node biopsy in large animals using direct image projection device", Proc. SPIE 9696, Molecular-Guided Surgery: Molecules, Devices, and Applications II, 96960K (4 March 2016); https://doi.org/10.1117/12.2209657

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available