Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus optical coherence microscopy

Szymon Tamborski; Hong Chou Lyu; Danuta Bukowska; Hubert Dolezyczek; Grzegorz Wilczynski; Daniel Szlag; Theo Lasser; Maciej Wojtkowski; Maciej Szkulmowski

doi:10.1117/12.2214779

8 March 2016 Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus optical coherence microscopy

Szymon Tamborski, Hong Chou Lyu, Danuta Bukowska, Hubert Dolezyczek, Grzegorz Wilczynski, Daniel Szlag, Theo Lasser, Maciej Wojtkowski, Maciej Szkulmowski

Author Affiliations +

Proceedings Volume 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX; 969718 (2016) https://doi.org/10.1117/12.2214779
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We used Optical Coherence Microscopy (OCM) to monitor structural and functional changes due to ischemic stroke in small animals brains in vivo. To obtain lateral resolution of 2.2 μm over the range of 600 μm we used extended focus configuration of OCM instrument involving Bessel beam. It provided access to detailed 3D information about the changes in brain vascular system up to the level of capillaries across I and II/III layers of neocortex. We used photothrombotic stroke model involving photoactive application of rose bengal to assure minimal invasiveness of the procedure and precise localization of the clot distribution center. We present the comparative analysis involving structural and angiographic maps of the stroke-affected brain enabling in-depth insight to the process of development of the disorder.

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Szymon Tamborski, Hong Chou Lyu, Danuta Bukowska, Hubert Dolezyczek, Grzegorz Wilczynski, Daniel Szlag, Theo Lasser, Maciej Wojtkowski, and Maciej Szkulmowski "Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus optical coherence microscopy", Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 969718 (8 March 2016); https://doi.org/10.1117/12.2214779

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