3 May 2016 Cellphone-based hand-held microplate reader for point-of-care ELISA testing (Conference Presentation)
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Abstract
Enzyme-linked immunosorbent assay (ELISA) in a microplate format has been a gold standard first-line clinical test for diagnosis of various diseases including infectious diseases. However, this technology requires a relatively large and expensive multi-well scanning spectrophotometer to read and quantify the signal from each well, hindering its implementation in resource-limited-settings. Here, we demonstrate a cost-effective and handheld smartphone-based colorimetric microplate reader for rapid digitization and quantification of immunoserology-related ELISA tests in a conventional 96-well plate format at the point of care (POC). This device consists of a bundle of 96 optical fibers to collect the transmitted light from each well of the microplate and direct all the transmission signals from the wells onto the camera of the mobile-phone. Captured images are then transmitted to a remote server through a custom-designed app, and both quantitative and qualitative diagnostic results are returned back to the user within ~1 minute per 96-well plate by using a machine learning algorithm. We tested this mobile-phone based micro-plate reader in a clinical microbiology lab using FDA-approved mumps IgG, measles IgG, and herpes simplex virus IgG (HSV-1 and HSV-2) ELISA tests on 1138 remnant patient samples (roughly 50% training and 50% testing), and achieved an overall accuracy of ~99% or higher for each ELISA test. This handheld and cost-effective platform could be immediately useful for large-scale vaccination monitoring in low-infrastructure settings, and also for other high-throughput disease screening applications at POC.
Conference Presentation
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Brandon Berg, Bingen Cortazar, Derek Tseng, Haydar Ozkan, Steve Feng, Qingshan Wei, Raymond Y. Chan, Jordi Burbano, Qamar Farooqui, Michael Lewinski, Dino Di Carlo, Omai B. Garner, Aydogan Ozcan, "Cellphone-based hand-held microplate reader for point-of-care ELISA testing (Conference Presentation)", Proc. SPIE 9699, Optics and Biophotonics in Low-Resource Settings II, 969905 (3 May 2016); doi: 10.1117/12.2209125; https://doi.org/10.1117/12.2209125
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