Fabrication of Indocyanine Green and 2H, 3H-perfluoropentane loaded microbubbles for fluorescence and ultrasound imaging

Yutong He; Qiang Wu; Rong Ma; Shufang Chang; Pengfei Shao; Ronald Xu

doi:10.1117/12.2211333

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10 March 2016 Fabrication of Indocyanine Green and 2H, 3H-perfluoropentane loaded microbubbles for fluorescence and ultrasound imaging

Yutong He, Qiang Wu, Rong Ma, Shufang Chang, Pengfei Shao, Ronald Xu

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Proceedings Volume 9701, Multimodal Biomedical Imaging XI; 97010T (2016) https://doi.org/10.1117/12.2211333
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

As a near-infrared (NIR) fluorescence dye, Indocyanine Green (ICG) has not gained broader clinical applications, owing to its multiple limitations such as concentration-dependent aggregation, low fluorescence quantum yield, poor physicochemical stability and rapid elimination from the body. In the meanwhile, 2H,3H-perfluoropentane (H-PFP) has been widely studied in ultrasound imaging as a vehicle for targeted delivery of contrast agents and drugs. We synthesized a novel dual-modal fluorescence and ultrasound contrast agent by encapsulating ICG and H-PFP in lipid microbubbles using a liquid-driven coaxial flow focusing (LDCFF) process. Uniform microbubbles with the sizes ranging from 1-10um and great ICG loading efficiency was achieved by this method. Our benchtop experiments showed that ICG/H-PFP microbubbles exhibited less aggregation, increased fluorescence intensity and more stable photostability compared to free ICG aqueous solution. Our phantom experiments demonstrated that ICG/H-PFP microbubbles enhanced the imaging contrasts in fluorescence imaging and ultrasonography. Our animal experiments indicated that ICG/H-PFP microbubbles extended the ICG life time and facilitated dual mode fluorescence and ultrasound imaging in vivo.

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Yutong He, Qiang Wu, Rong Ma, Shufang Chang, Pengfei Shao, and Ronald Xu "Fabrication of Indocyanine Green and 2H, 3H-perfluoropentane loaded microbubbles for fluorescence and ultrasound imaging", Proc. SPIE 9701, Multimodal Biomedical Imaging XI, 97010T (10 March 2016); https://doi.org/10.1117/12.2211333

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