Line-scanning, stage scanning confocal microscope

John A. Carucci; Mary Stevenson; Daniel Gareau

doi:10.1117/12.2218302

11 March 2016 Line-scanning, stage scanning confocal microscope

John A. Carucci, Mary Stevenson, Daniel Gareau

Proceedings Volume 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis; 97030F (2016) https://doi.org/10.1117/12.2218302
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We created a line-scanning, stage scanning confocal microscope as part of a new procedure: video assisted micrographic surgery (VAMS). The need for rapid pathological assessment of the tissue on the surface of skin excisions very large since there are 3.5 million new skin cancers diagnosed annually in the United States. The new design presented here is a confocal microscope without any scanning optics. Instead, a line is focused in space and the sample, which is flattened, is physically translated such that the line scans across its face in a direction perpendicular to the line its self. The line is 6mm long and the stage is capable of scanning 50 mm, hence the field of view is quite large. The theoretical diffraction-limited resolution is 0.7um lateral and 3.7um axial. However, in this preliminary report, we present initial results that are a factor of 5-7 poorer in resolution. The results are encouraging because they demonstrate that the linear array detector measures sufficient signal from fluorescently labeled tissue and also demonstrate the large field of view achievable with VAMS.

Conference Presentation

Citation Download Citation

John A. Carucci, Mary Stevenson, and Daniel Gareau "Line-scanning, stage scanning confocal microscope", Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 97030F (11 March 2016); https://doi.org/10.1117/12.2218302

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