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We explored the depth penetration in tissue-mimicking intralipid-based phantoms in SWIR (800-1650 nm) using a hyperspectral imaging system composed from a 2D CCD camera coupled to a microscope. Hyperspectral images in transmission and reflection geometries were collected with a spectral resolution of 5.27 nm and a total acquisition time of 3 minutes or less that minimized artifacts from sample drying. Michelson spatial contrast was used as a metric to evaluate light penetration. Results from both transmission and reflection geometries consistently revealed the highest spatial contrast in the wavelength range of 1300 to 1350 nm.
Hairong Zhang,Daniel Salo,David M. Kim, andMikhail Y. Berezin
"Penetration depth in tissue-mimicking phantoms from hyperspectral imaging in SWIR in transmission and reflection geometry", Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 970311 (7 March 2016); https://doi.org/10.1117/12.2213754
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Hairong Zhang, Daniel Salo, David M. Kim, Mikhail Y. Berezin, "Penetration depth in tissue-mimicking phantoms from hyperspectral imaging in SWIR in transmission and reflection geometry," Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 970311 (7 March 2016); https://doi.org/10.1117/12.2213754