7 March 2016 Real-time quantum cascade laser-based infrared microspectroscopy in-vivo
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Abstract
Infrared microscopy can be performed to observe dynamic processes on a microscopic scale. Fourier-transform infrared spectroscopy-based microscopes are bound to limitations regarding time resolution, which hampers their potential for imaging fast moving systems. In this manuscript we present a quantum cascade laser-based infrared microscope which overcomes these limitations and readily achieves standard video frame rates. The capabilities of our setup are demonstrated by observing dynamical processes at their specific time scales: fermentation, slow moving Amoeba Proteus and fast moving Caenorhabditis elegans. Mid-infrared sampling rates between 30 min and 20 ms are demonstrated.
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N. Kröger-Lui, N. Kröger-Lui, K. Haase, K. Haase, A. Pucci, A. Pucci, A. Schönhals, A. Schönhals, W. Petrich, W. Petrich, "Real-time quantum cascade laser-based infrared microspectroscopy in-vivo", Proc. SPIE 9704, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry, 97040J (7 March 2016); doi: 10.1117/12.2213239; https://doi.org/10.1117/12.2213239
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