7 March 2016 Dental caries imaging using hyperspectral stimulated Raman scattering microscopy
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Abstract
We report the development of a polarization-resolved hyperspectral stimulated Raman scattering (SRS) imaging technique based on a picosecond (ps) laser-pumped optical parametric oscillator system for label-free imaging of dental caries. In our imaging system, hyperspectral SRS images (512×512 pixels) in both fingerprint region (800-1800 cm-1) and high-wavenumber region (2800-3600 cm-1) are acquired in minutes by scanning the wavelength of OPO output, which is a thousand times faster than conventional confocal micro Raman imaging. SRS spectra variations from normal enamel to caries obtained from the hyperspectral SRS images show the loss of phosphate and carbonate in the carious region. While polarization-resolved SRS images at 959 cm-1 demonstrate that the caries has higher depolarization ratio. Our results demonstrate that the polarization resolved-hyperspectral SRS imaging technique developed allows for rapid identification of the biochemical and structural changes of dental caries.
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Zi Wang, Zi Wang, Wei Zheng, Wei Zheng, Lin Jian, Lin Jian, Zhiwei Huang, Zhiwei Huang, "Dental caries imaging using hyperspectral stimulated Raman scattering microscopy", Proc. SPIE 9704, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry, 97040M (7 March 2016); doi: 10.1117/12.2213793; https://doi.org/10.1117/12.2213793
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