7 March 2016 FT-IR spectroscopy characterization of schwannoma: a case study
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Abstract
Schwannoma are rare benign neural neoplasia. The clinical diagnosis could be improved if novel optical techniques are performed. Among these techniques, FT-IR is one of the currently techniques which has been applied for samples discrimination using biochemical information with minimum sample preparation. In this work, we report a case of a schwannoma in the cervical region. A histological examination described a benign process. An immunohistochemically examination demonstrated positivity to anti-S100 protein antibody, indicating a diagnosis of schwannoma. The aim of this analysis was to characterize FT-IR spectrum of the neoplastic and normal tissue in the fingerprint (1000-1800 cm-1) and high wavenumber region (2800-3600 cm-1). The IR spectra were collect from tumor tissue and normal nerve samples by a FT-IR spectrophotometer (Spotlight Perkin Elmer 400, USA) with 64 scans, and resolution of 4 cm−1. A total of twenty spectra were recorded (10 from schwannoma and 10 from nerve). Multivariate Analysis was used to classify the data. Through average and standard deviation analysis we observed that the main spectral change occurs at ≈1600 cm-1 (amide I) and ≈1400 cm-1 (amide III) in the fingerprint region, and in CH2/CH3 protein-lipids and OH-water vibrations for the high wavenumber region. In conclusion, FT-IR could be used as a technique for schwannoma analysis helping to establish specific diagnostic.
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Isabelle Ferreira, Isabelle Ferreira, Lazaro P. M. Neto, Lazaro P. M. Neto, Maurilio José das Chagas, Maurilio José das Chagas, Luís Felipe C. S. Carvalho, Luís Felipe C. S. Carvalho, Laurita dos Santos, Laurita dos Santos, Marcelo Ribas, Marcelo Ribas, Vinicius Loddi, Vinicius Loddi, Airton A. Martin, Airton A. Martin, } "FT-IR spectroscopy characterization of schwannoma: a case study", Proc. SPIE 9704, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry, 97040Z (7 March 2016); doi: 10.1117/12.2212557; https://doi.org/10.1117/12.2212557
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