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21 March 2016 Microfluidic in-channel multi-electrode platform for neurotransmitter sensing
A. Kara, J. Mathault, A. Reitz, M. Boisvert, F. Tessier, J. Greener, A. Miled
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Proceedings Volume 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV; 97050X (2016) https://doi.org/10.1117/12.2211763
Event: SPIE BiOS, 2016, San Francisco, California, United States
Abstract
In this project we present a microfluidic platform with in-channel micro-electrodes for in situ screening of bio/chemical samples through a lab-on-chip system. We used a novel method to incorporate electrochemical sensors array (16x20) connected to a PCB, which opens the way for imaging applications. A 200 μm height microfluidic channel was bonded to electrochemical sensors. The micro-channel contains 3 inlets used to introduce phosphate buffer saline (PBS), ferrocynide and neurotransmitters. The flow rate was controlled through automated micro-pumps. A multiplexer was used to scan electrodes and perform individual cyclic voltammograms by a custom potentiostat. The behavior of the system was linear in terms of variation of current versus concentration. It was used to detect the neurotransmitters serotonin, dopamine and glutamate.
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A. Kara, J. Mathault, A. Reitz, M. Boisvert, F. Tessier, J. Greener, and A. Miled "Microfluidic in-channel multi-electrode platform for neurotransmitter sensing", Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 97050X (21 March 2016); https://doi.org/10.1117/12.2211763
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KEYWORDS
Electrodes
Microfluidics
Neurotransmitters
Multiplexers
Potassium
Brain
Calibration
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