21 March 2016 Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis
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Abstract
Signal transductions including multiple protein post-translational modifications (PTM), protein-protein interactions (PPI), and protein-nucleic acid interaction (PNI) play critical roles for cell proliferation and differentiation that are directly related to the cancer biology. Traditional methods, like mass spectrometry, immunoprecipitation, fluorescence resonance energy transfer, and fluorescence correlation spectroscopy require a large amount of sample and long processing time. “microchannel for multiple-parameter analysis of proteins in single-complex (mMAPS)”we proposed can reduce the process time and sample volume because this system is composed by microfluidic channels, fluorescence microscopy, and computerized data analysis. In this paper, we will present an automated mMAPS including integrated microfluidic device, automated stage and electrical relay for high-throughput clinical screening. Based on this result, we estimated that this automated detection system will be able to screen approximately 150 patient samples in a 24-hour period, providing a practical application to analyze tissue samples in a clinical setting.
Conference Presentation
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Po-Jung Huang, Po-Jung Huang, Sina Baghbani Kordmahale, Sina Baghbani Kordmahale, Chao-Kai Chou, Chao-Kai Chou, Hirohito Yamaguchi, Hirohito Yamaguchi, Mien-Chie Hung, Mien-Chie Hung, Jun Kameoka, Jun Kameoka, "Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis", Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 970511 (21 March 2016); doi: 10.1117/12.2213259; https://doi.org/10.1117/12.2213259
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