21 March 2016 Development of microfluidic-based cell collection devices for in vitro and in vivo use
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The NANIVID – or Nano Intravital Device – is an implantable delivery tool designed to locally affect the tumor microenvironment in vivo. This technology is being redesigned and validated as a cell collection tool for the study of metastatic cancer cells. A methodology has been developed to facilitate this transition, consisting of microfluidic analysis of the device microchannels and a series of cell-related collection experiments building up to in vivo collection. Single-chamber designs were first used to qualitatively demonstrate the feasibility of cell collection ex vivo. This was followed by the development and implementation of devices containing a second, negative-control chamber for quantitative analysis. This work sets the foundation for in vivo cancer cell migration studies utilizing the NANIVID.
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Logan Butt, Logan Butt, Dave Entenberg, Dave Entenberg, L. P. Madhubhani Hemachandra, L. P. Madhubhani Hemachandra, Matthew Strohmayer, Matthew Strohmayer, Patricia Keely, Patricia Keely, Julio Aguirre-Ghiso, Julio Aguirre-Ghiso, John S. Condeelis, John S. Condeelis, James Castracane, James Castracane, "Development of microfluidic-based cell collection devices for in vitro and in vivo use", Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 97051A (21 March 2016); doi: 10.1117/12.2213316; https://doi.org/10.1117/12.2213316

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