21 March 2016 Development of microfluidic-based cell collection devices for in vitro and in vivo use
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Abstract
The NANIVID – or Nano Intravital Device – is an implantable delivery tool designed to locally affect the tumor microenvironment in vivo. This technology is being redesigned and validated as a cell collection tool for the study of metastatic cancer cells. A methodology has been developed to facilitate this transition, consisting of microfluidic analysis of the device microchannels and a series of cell-related collection experiments building up to in vivo collection. Single-chamber designs were first used to qualitatively demonstrate the feasibility of cell collection ex vivo. This was followed by the development and implementation of devices containing a second, negative-control chamber for quantitative analysis. This work sets the foundation for in vivo cancer cell migration studies utilizing the NANIVID.
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Logan Butt, Logan Butt, Dave Entenberg, Dave Entenberg, L. P. Madhubhani Hemachandra, L. P. Madhubhani Hemachandra, Matthew Strohmayer, Matthew Strohmayer, Patricia Keely, Patricia Keely, Julio Aguirre-Ghiso, Julio Aguirre-Ghiso, John S. Condeelis, John S. Condeelis, James Castracane, James Castracane, "Development of microfluidic-based cell collection devices for in vitro and in vivo use", Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 97051A (21 March 2016); doi: 10.1117/12.2213316; https://doi.org/10.1117/12.2213316
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