Chromatically encoded high-speed photography of cavitation bubble dynamics inside inhomogeneous ophthalmic tissue

N. Tinne; B. Matthias; F. Kranert; C. Wetzel; A. Krüger; T. Ripken

doi:10.1117/12.2211042

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7 March 2016 Chromatically encoded high-speed photography of cavitation bubble dynamics inside inhomogeneous ophthalmic tissue

N. Tinne, B. Matthias, F. Kranert, C. Wetzel, A. Krüger, T. Ripken

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Proceedings Volume 9706, Optical Interactions with Tissue and Cells XXVII; 97060R (2016) https://doi.org/10.1117/12.2211042
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The interaction effect of photodisruption, which is used for dissection of biological tissue with fs-laser pulses, has been intensively studied inside water as prevalent sample medium. In this case, the single effect is highly reproducible and, hence, the method of time-resolved photography is sufficiently applicable. In contrast, the reproducibility significantly decreases analyzing more solid and anisotropic media like biological tissue. Therefore, a high-speed photographic approach is necessary in this case. The presented study introduces a novel technique for high-speed photography based on the principle of chromatic encoding. For illumination of the region of interest within the sample medium, the light paths of up to 12 LEDs with various emission wavelengths are overlaid via optical filters. Here, MOSFET-electronics provide a LED flash with a duration <100 ns; the diodes are externally triggered with a distinct delay for every LED. Furthermore, the different illumination wavelengths are chromatically separated again for detection via camera chip. Thus, the experimental setup enables the generation of a time-sequence of ≤ 12 images of a single cavitation bubble dynamics. In comparison to conventional time-resolved photography, images in sample media like water and HEMA show the significant advantages of this novel illumination technique. In conclusion, the results of this study are of great importance for the fundamental evaluation of the laser-tissue interaction inside anisotropic biological tissue and for the optimization of the surgical process with high-repetition rate fs-lasers. Additionally, this application is also suitable for the investigation of other microscopic, ultra-fast events in transparent inhomogeneous materials.

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N. Tinne, B. Matthias, F. Kranert, C. Wetzel, A. Krüger, and T. Ripken "Chromatically encoded high-speed photography of cavitation bubble dynamics inside inhomogeneous ophthalmic tissue", Proc. SPIE 9706, Optical Interactions with Tissue and Cells XXVII, 97060R (7 March 2016); https://doi.org/10.1117/12.2211042

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