Transmittance of MCF-7 breast tumor cell line through visible and near infrared spectrum

H. Özgür Tabakoğlu

doi:10.1117/12.2212647

7 March 2016 Transmittance of MCF-7 breast tumor cell line through visible and near infrared spectrum

H. Özgür Tabakoğlu

Author Affiliations +

Proceedings Volume 9706, Optical Interactions with Tissue and Cells XXVII; 970619 (2016) https://doi.org/10.1117/12.2212647
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

In this study, light transmittance of MCF-7 tumor cells from 450 nm to 1100 nm has been measured in their growing medium and evaluated. Transmittance differences have been tried to be put forward in cancer cell line on visible (VIS) and near infrared (NIR) spectrum as well as in between different numbers of cells in medium. An absorption-reflection spectrophotometer was used in the experiments. System has a tungsten light source, optical chopper, a monochromator, sample chamber, silicon detectors, lock-in amplifier and computer. System was controlled by software in order to adjust scan range, scan steps and grating configuration. Cells were grown in medium, and measurements were taken from cells while they were in 5 ml medium. According to our findings, there are significant differences between VIS and NIR regions for the same number of cells. There were found no statistical difference among different numbers of cells. Increasing number of cells has not affected the transmittance. Transmittance of medium is not significantly different from different concentration of cells.

Conference Presentation

Citation Download Citation

H. Özgür Tabakoğlu "Transmittance of MCF-7 breast tumor cell line through visible and near infrared spectrum", Proc. SPIE 9706, Optical Interactions with Tissue and Cells XXVII, 970619 (7 March 2016); https://doi.org/10.1117/12.2212647

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