Biodynamic profiling of three-dimensional tissue growth techniques

Hao Sun; Dan Merrill; John Turek; David Nolte

doi:10.1117/12.2214309

17 March 2016 Biodynamic profiling of three-dimensional tissue growth techniques

Hao Sun, Dan Merrill, John Turek, David Nolte

Proceedings Volume 9707, Dynamics and Fluctuations in Biomedical Photonics XIII; 97070S (2016) https://doi.org/10.1117/12.2214309
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Three-dimensional tissue culture presents a more biologically relevant environment in which to perform drug development than conventional two-dimensional cell culture. However, obtaining high-content information from inside three dimensional tissue has presented an obstacle to rapid adoption of 3D tissue culture for pharmaceutical applications. Biodynamic imaging is a high-content three-dimensional optical imaging technology based on low-coherence interferometry and digital holography that uses intracellular dynamics as high-content image contrast. In this paper, we use biodynamic imaging to compare pharmaceutical responses to Taxol of three-dimensional multicellular spheroids grown by three different growth techniques: rotating bioreactor, hanging-drop and plate-grown spheroids. The three growth techniques have systematic variations among tissue cohesiveness and intracellular activity and consequently display different pharmacodynamics under identical drug dose conditions. The in vitro tissue cultures are also compared to ex vivo living biopsies. These results demonstrate that three-dimensional tissue cultures are not equivalent, and that drug-response studies must take into account the growth method.

Conference Presentation

Citation Download Citation

Hao Sun, Dan Merrill, John Turek, and David Nolte "Biodynamic profiling of three-dimensional tissue growth techniques", Proc. SPIE 9707, Dynamics and Fluctuations in Biomedical Photonics XIII, 97070S (17 March 2016); https://doi.org/10.1117/12.2214309

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