Combined label-free optical and optoacoustic imaging of model organisms at mesoscopy and microscopy resolutions

Dominik Soliman; George J. Tserevelakis; Murad Omar; Vasilis Ntziachristos

doi:10.1117/12.2208861

15 March 2016 Combined label-free optical and optoacoustic imaging of model organisms at mesoscopy and microscopy resolutions

Dominik Soliman, George J. Tserevelakis, Murad Omar, Vasilis Ntziachristos

Author Affiliations +

Proceedings Volume 9708, Photons Plus Ultrasound: Imaging and Sensing 2016; 97083B (2016) https://doi.org/10.1117/12.2208861
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We present a multi-scale imaging system that integrates five optoacoustic and multi-photon modalities into the same device. The hybrid microscope offers a unique zoom-in ability by allowing for optoacoustic microscopy and mesoscopy scans of the sample within the same imaging framework. Furthermore, by combining several label-free modalities, we are able to visualize a broad range of anatomical features, taking advantage of their complementary contrast mechanisms. We characterize the spatial resolution and relative orientation of the different sub-modalities and demonstrate the system’s performance by the imaging of several model organisms ex vivo. The presented ability to dynamically vary scanning volume and resolution together with its multi-contrast and label-free imaging capabilities make the hybrid microscope a promising tool for comprehensive biological imaging.

Citation Download Citation

Dominik Soliman, George J. Tserevelakis, Murad Omar, and Vasilis Ntziachristos "Combined label-free optical and optoacoustic imaging of model organisms at mesoscopy and microscopy resolutions", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97083B (15 March 2016); https://doi.org/10.1117/12.2208861

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