15 March 2016 In vivo switchable optical- and acoustic-resolution photoacoustic microscopy
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Photoacoustic microscopy (PAM) provides high resolution and large penetration depth by utilizing the high optical sensitivity and low scattering of ultrasound. Hybrid PAM systems can be classified into two categories: opticalresolution photoacoustic microscopy (OR-PAM) and acoustic-resolution photoacoustic microscopy (AR-PAM). ORPAM provides a very high lateral resolution with a strong optical focus, but the penetration depth is limited to one optical transport mean free path. AR-PAM provides a relatively greater penetration depth using diffused light in biological tissues. The resolution of AR-PAM is determined by its ultrasonic parameters. In this study, we performed an in vivo testing of a switchable OR-/AR-PAM system. In this system, two modes can be switched by changing its collimator lens and optical fiber. The lateral resolution of OR-PAM was measured using a resolution test target, and the full width at half maximum (FWHM) of the edge spread function was 2.5 μm. To calculate the lateral resolution of ARPAM, a 6-μm-diameter carbon fiber was used, and the FWHM of the line spread function was 80.2 μm. We successfully demonstrated the multiscale imaging capability of the switchable OR-/AR-PAM system by visualizing microvascular networks in mouse ears, brain, legs, skin, and eyes.
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Seungwan Jeon, Seungwan Jeon, Jaewoo Kim, Jaewoo Kim, Chulhong Kim, Chulhong Kim, } "In vivo switchable optical- and acoustic-resolution photoacoustic microscopy", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 970845 (15 March 2016); doi: 10.1117/12.2212199; https://doi.org/10.1117/12.2212199

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