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15 March 2016 To assess the reparative ability of differentiated mesenchymal stem cells in a rat critical size bone repair defect model using high frequency co-registered photoacoustic/ultrasound imaging and micro computed tomography
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Abstract
Stem cell based treatments hold great potential and promise to address many unmet clinical needs. The importance of non-invasive imaging techniques to monitor transplanted stem cells qualitatively and quantitatively is crucial. The objective of this study was to create a critical size bone defect in the rat femur and then assess the ability of the differentiated mesenchymal stem cells (MSCs) to repair the defect using high frequency co-registered photoacoustic(PA)/ultrasound(US) imaging and micro computed tomography (μCT) over an 8 week period. Combined PA and US imaging was performed using 256 elements, 21 MHz frequency linear-array transducer combined with multichannel collecting system. In vivo 3D PA and US images of the defect bone in the rat femur were acquired after 4 and 8 weeks of the surgery. 3D co-registered structural such as microvasculature and the functional images such as total concentration of haemoglobin (HbT) and the haemoglobin oxygen saturation (sO2) were obtained using PA and US imaging. Bone formation was assessed after 4 and 8 weeks of the surgery by μCT. High frequency linear-array based coregistered PA/US imaging has been found promising in terms of non-invasiveness, sensitivity, adaptability, high spatial and temporal resolution at sufficient depths for the assessment of the reparative ability of MSCs in a rat critical size bone repair defect model.
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Haroon Zafar, Sean Gaynard, Cathal Ó Flatharta, Tatiana Doroshenkova, Declan Devine, Faisal Sharif, Frank Barry, Jessica Hayes, Mary Murphy, and Martin J. Leahy "To assess the reparative ability of differentiated mesenchymal stem cells in a rat critical size bone repair defect model using high frequency co-registered photoacoustic/ultrasound imaging and micro computed tomography", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 970853 (15 March 2016); https://doi.org/10.1117/12.2211382
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