Mechanical characterization of the mouse diaphragm with optical coherence elastography reveals fibrosis-related change of direction-dependent muscle tissue stiffness

Shang Wang; James A. Loehr; Irina V. Larina; George G. Rodney Jr.; Kirill V. Larin

doi:10.1117/12.2211160

9 March 2016 Mechanical characterization of the mouse diaphragm with optical coherence elastography reveals fibrosis-related change of direction-dependent muscle tissue stiffness

Shang Wang, James A. Loehr, Irina V. Larina, George G. Rodney Jr., Kirill V. Larin

Author Affiliations +

Proceedings Volume 9710, Optical Elastography and Tissue Biomechanics III; 97100P (2016) https://doi.org/10.1117/12.2211160
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The diaphragm, composed of skeletal muscle, plays an important role in respiration through its dynamic contraction. Genetic and molecular studies of the biomechanics of mouse diaphragm can provide great insights into an improved understanding and potential treatment of the disorders that lead to diaphragm dysfunction (i.e. muscular dystrophy). However, due to the small tissue size, mechanical assessment of mouse diaphragm tissue under its proper physiological conditions has been challenging. Here, we present the application of noncontact optical coherence elastography (OCE) for quantitative elastic characterization of ex vivo mouse diaphragm. Phase-sensitive optical coherence tomography was combined with a focused air-puff system to capture and measure the elastic wave propagation from tissue surface. Experiments were performed on wildtype and dystrophic mouse diaphragm tissues containing different levels of fibrosis. The OCE measurements of elastic wave propagation were conducted along both the longitudinal and transverse axis of the muscle fibers. Cross-correlation of the temporal displacement profiles from different spatial locations was utilized to obtain the propagation time delay, which was used to calculate the wave group velocity and to further quantify the tissue Young’s modulus. Prior to and after OCE assessment, peak tetanic force was measured to monitor viability of the tissue during the elasticity measurements. Our experimental results indicate a positive correlation between fibrosis level and tissue stiffness, suggesting this elastic-wave-based OCE method could be a useful tool to monitor mechanical properties of skeletal muscle under physiological and pathological conditions.

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Shang Wang, James A. Loehr, Irina V. Larina, George G. Rodney Jr., and Kirill V. Larin "Mechanical characterization of the mouse diaphragm with optical coherence elastography reveals fibrosis-related change of direction-dependent muscle tissue stiffness", Proc. SPIE 9710, Optical Elastography and Tissue Biomechanics III, 97100P (9 March 2016); https://doi.org/10.1117/12.2211160

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KEYWORDS

Tissues

Tissue optics

Elastography

Optical coherence tomography

Wave propagation

Coherence (optics)

Optical fibers

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