Cell sheets image validation of phase-diversity homodyne OCT and effect of the light irradiation on cells

Naoko Senda; Kentaro Osawa

doi:10.1117/12.2205331

6 April 2016 Cell sheets image validation of phase-diversity homodyne OCT and effect of the light irradiation on cells

Naoko Senda, Kentaro Osawa

Proceedings Volume 9711, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 97110F (2016) https://doi.org/10.1117/12.2205331
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Optical coherence tomography (OCT) is one of powerful 3D tissue imaging tools with no fluorescence staining. We have reported that Phase-Diversity Homodyne OCT developed in Hitachi could be useful for non-invasive regeneration tissue evaluation test. The OCT enables cell imaging because of high resolution (axial resolution; ~2.6 μm, lateral resolution; ~1 μm, in the air), whereas conventional OCT was not used for cell imaging because of low resolution (10~20 μm). Furthermore, the OCT has advantage over other 3D imaging devices in cost because the light source and the objective were originally used as an optical pickup of compact disc. In this report, we aimed to assess effectiveness and safety of Phase-Diversity Homodyne OCT cell imaging. Effectiveness of OCT was evaluated by imaging a living cell sheet of human oral mucosal epithelial cells. OCT images were compared with reflection confocal microscopy (RCM) images, because confocal optical system is the highest resolution (<1 μm) 3D in vivo imaging technique. Similar nuclei images were confirmed with OCT and RCM, which suggested the OCT has enough resolution to image nuclei inside a cell sheet. Degree of differentiation could be estimated using OCT images, which becomes possible because the size of cells depends on distribution of differentiation. Effect of the OCT light irradiation on cells was studied using NIH/3T3 cells. Light irradiation, the exposure amount of which is equivalent to OCT, had no impact on cell shape, cell viability, and proliferation rate. It suggested that the light irradiation has no cell damage under the condition.

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Naoko Senda and Kentaro Osawa "Cell sheets image validation of phase-diversity homodyne OCT and effect of the light irradiation on cells", Proc. SPIE 9711, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 97110F (6 April 2016); https://doi.org/10.1117/12.2205331

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KEYWORDS

Optical coherence tomography

Image resolution

Homodyne detection

Confocal microscopy

Tissues

Semiconductor lasers

Stereoscopy

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