6 April 2016 Whole slide imaging of unstained tissue using lensfree microscopy
Author Affiliations +
Pathologist examination of tissue slides provides insightful information about a patient’s disease. Traditional analysis of tissue slides is performed under a binocular microscope, which requires staining of the sample and delays the examination. We present a simple cost-effective lensfree imaging method to record 2–4μm resolution wide-field (10 mm2 to 6 cm2) images of unstained tissue slides. The sample processing time is reduced as there is no need for staining. A wide field of view (10 mm2) lensfree hologram is recorded in a single shot and the image is reconstructed in 2s providing a very fast acquisition chain. The acquisition is multispectral, i.e. multiple holograms are recorded simultaneously at three different wavelengths, and a dedicated holographic reconstruction algorithm is used to retrieve both amplitude and phase. Whole tissue slides imaging is obtained by recording 130 holograms with X-Y translation stages and by computing the mosaic of a 25 x 25 mm2 reconstructed image. The reconstructed phase provides a phase-contrast-like image of the unstained specimen, revealing structures of healthy and diseased tissue. Slides from various organs can be reconstructed, e.g. lung, colon, ganglion, etc. To our knowledge, our method is the first technique that enables fast wide-field lensfree imaging of such unlabeled dense samples. This technique is much cheaper and compact than a conventional phase contrast microscope and could be made portable. In sum, we present a new methodology that could quickly provide useful information when a rapid diagnosis is needed, such as tumor margin identification on frozen section biopsies during surgery.
Conference Presentation
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sophie Nhu An Morel, Sophie Nhu An Morel, Lionel Hervé, Lionel Hervé, Thomas Bordy, Thomas Bordy, Olivier Cioni, Olivier Cioni, Antoine Delon, Antoine Delon, Catherine Fromentin, Catherine Fromentin, Jean-Marc Dinten, Jean-Marc Dinten, Cédric Allier, Cédric Allier, } "Whole slide imaging of unstained tissue using lensfree microscopy", Proc. SPIE 9711, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 97111L (6 April 2016); doi: 10.1117/12.2210831; https://doi.org/10.1117/12.2210831

Back to Top