Phenotype classification of single cells using SRS microscopy, RNA sequencing, and microfluidics
(Conference Presentation)

Aaron M. Streets; Chen Cao; Xiannian Zhang; Yanyi Huang

doi:10.1117/12.2214702

27 April 2016 Phenotype classification of single cells using SRS microscopy, RNA sequencing, and microfluidics (Conference Presentation)

Aaron M. Streets, Chen Cao, Xiannian Zhang, Yanyi Huang

Author Affiliations +

Proceedings Volume 9712, Multiphoton Microscopy in the Biomedical Sciences XVI; 971209 (2016) https://doi.org/10.1117/12.2214702
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Phenotype classification of single cells reveals biological variation that is masked in ensemble measurement. This heterogeneity is found in gene and protein expression as well as in cell morphology. Many techniques are available to probe phenotypic heterogeneity at the single cell level, for example quantitative imaging and single-cell RNA sequencing, but it is difficult to perform multiple assays on the same single cell. In order to directly track correlation between morphology and gene expression at the single cell level, we developed a microfluidic platform for quantitative coherent Raman imaging and immediate RNA sequencing (RNA-Seq) of single cells. With this device we actively sort and trap cells for analysis with stimulated Raman scattering microscopy (SRS). The cells are then processed in parallel pipelines for lysis, and preparation of cDNA for high-throughput transcriptome sequencing. SRS microscopy offers three-dimensional imaging with chemical specificity for quantitative analysis of protein and lipid distribution in single cells. Meanwhile, the microfluidic platform facilitates single-cell manipulation, minimizes contamination, and furthermore, provides improved RNA-Seq detection sensitivity and measurement precision, which is necessary for differentiating biological variability from technical noise. By combining coherent Raman microscopy with RNA sequencing, we can better understand the relationship between cellular morphology and gene expression at the single-cell level.

Conference Presentation

Citation Download Citation

Aaron M. Streets, Chen Cao, Xiannian Zhang, and Yanyi Huang "Phenotype classification of single cells using SRS microscopy, RNA sequencing, and microfluidics (Conference Presentation)", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971209 (27 April 2016); https://doi.org/10.1117/12.2214702

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KEYWORDS

Microscopy

Microfluidics

Chemical analysis

Coherence imaging

Proteins

Raman spectroscopy

3D image processing

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