14 March 2016 Nonlinear optical Stokes ellipsometric (NOSE) microscopy for imaging the nonlinear susceptibility tensors of collagen
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Abstract
Nonlinear optical Stokes ellipsometric (NOSE) microscopy was demonstrated for the analysis of collagen structure in a mouse tail section. NOSE is based on polarization-dependent second harmonic generation (SHG) imaging. The fast polarization-modulation was achieved using an electro-optic modulator (EOM), allowing for the potential of video-rate NOSE analysis. The signal to noise advantages associated with suppression of 1/f noise by rapid polarization modulation allowed reliable recovery of the local-frame tensor on a per-pixel basis. An iterative approach involving laboratory to local frame coordinate transformation was developed to recover the spatial distribution of local-frame nonlinear susceptibility tensor elements of collagen as well as the polar and azimuthal orientation angles of the collagen structure.
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Ximeng Y. Dow, Ximeng Y. Dow, Emma L. DeWalt, Emma L. DeWalt, Shane Z. Sullivan, Shane Z. Sullivan, Paul D. Schmitt, Paul D. Schmitt, Garth J. Simpson, Garth J. Simpson, } "Nonlinear optical Stokes ellipsometric (NOSE) microscopy for imaging the nonlinear susceptibility tensors of collagen", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97121S (14 March 2016); doi: 10.1117/12.2212944; https://doi.org/10.1117/12.2212944
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