14 March 2016 The nature of multiphoton fluorescence from red blood cells
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Abstract
We report on the nature of multiphoton excited fluorescence observed from human erythrocytes (red blood cells RBC's) and their "ghosts" following 800nm sub-15 fs excitation. The detected optical signal is assigned as two-photon excited fluorescence from hemoglobin. Our findings are supported by wavelength-resolved fluorescence lifetime decay measurements using time-correlated single photon counting system from RBC's, their ghosts as well as in vitro samples of various fluorophores including riboflavin, NADH, NAD(P)H, hemoglobin. We find that low-energy and short-duration pulses allow two-photon imaging of RBC’s, but longer more intense pulses lead to their destruction.
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Ilyas Saytashev, Ilyas Saytashev, Michael Murphy, Michael Murphy, Sam Osseiran, Sam Osseiran, Dana M. Spence, Dana M. Spence, Conor L. Evans, Conor L. Evans, Marcos Dantus, Marcos Dantus, "The nature of multiphoton fluorescence from red blood cells", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97121W (14 March 2016); doi: 10.1117/12.2213422; https://doi.org/10.1117/12.2213422
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