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14 March 2016 Mapping of intracellular concentrations of macromolecules by two-photon excited fluorescence lifetime imaging
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Abstract
Measurements and monitoring of concentrations of macromolecules in live cells and sub-cellular structures is of tremendous interest in cell biology and translational medicine. In this report we demonstrate a breakthrough potential of FLIM for real-time quantitative mapping of macromolecular distribution in the cell. In our approach we exploit a correlation existing between the fluorescence lifetime of fluorophores, refractive index and local concentrations of cellular macromolecules in the of fluorophore's microenvironment. We show a value of our approach for fundamental cell science and cellular diagnostic assays.
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Lixin Liu, Artem Pliss, Xiao Peng, Andrey Kuzmin, Junle Qu, and Paras N. Prasad "Mapping of intracellular concentrations of macromolecules by two-photon excited fluorescence lifetime imaging", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971221 (14 March 2016); https://doi.org/10.1117/12.2211889
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