Optically sectioned wide-field fluorescence lifetime imaging endoscopy enabled by structured illumination
(Conference Presentation)

Taylor Hinsdale; Bilal H. Malik; Jose J. Rico-Jimenez; Javier A. Jo; Kristen C. Maitland

doi:10.1117/12.2213599

27 April 2016 Optically sectioned wide-field fluorescence lifetime imaging endoscopy enabled by structured illumination (Conference Presentation)

Taylor Hinsdale, Bilal H. Malik, Jose J. Rico-Jimenez, Javier A. Jo, Kristen C. Maitland

Author Affiliations +

Proceedings Volume 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII; 97130X (2016) https://doi.org/10.1117/12.2213599
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We present a wide-field fluorescence lifetime imaging (FLIM) system with optical sectioning by structured illumination microscopy (SIM). FLIM measurements were made using a time gated ICCD camera in conjunction with a pulsed nitrogen dye laser operating at 450 nm. Intensity images were acquired at multiple time delays from a trigger initiated by a laser pulse to create a wide-field FLIM image, which was then combined with three phase SIM to provide optical sectioning. Such a mechanism has the potential to increase the reliability and accuracy of the FLIM measurements by rejecting background intensity. SIM also provides the opportunity to create volumetric FLIM images with the incorporation of scanning mechanisms for the sample plane. We present multiple embodiments of such a system: one as a free space endoscope and the other as a fiber microendoscope enabled by the introduction of a fiber bundle. Finally, we demonstrate the efficacy of such an imaging system by imaging dyes embedded in a tissue phantom.

Conference Presentation

Citation Download Citation

Taylor Hinsdale, Bilal H. Malik, Jose J. Rico-Jimenez, Javier A. Jo, and Kristen C. Maitland "Optically sectioned wide-field fluorescence lifetime imaging endoscopy enabled by structured illumination (Conference Presentation)", Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 97130X (27 April 2016); https://doi.org/10.1117/12.2213599

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Fluorescence lifetime imaging

Imaging systems

Endoscopy

Microscopy

Tissue optics

Cameras

Dye lasers

Show All Keywords

Keywords/Phrases

Search In:

Publication Years