Preliminary results obtained using a Lissajous trajectory beam-scanning approach coupled with temporal multiplexing by the implementation of an optical delay line demonstrate the ability to acquire 2 distinct focal planes simultaneously at frame rates >450 Hz for full 512 × 512 images. The use of multi-channel data acquisition cards allows for simultaneous multimodal image acquisition with perfect image registry between all imaging modalities. Also discussed here is the implementation of Lissajous trajectory beam-scanning on commercially available microscope hardware.
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Justin A. Newman, Shane Z. Sullivan, Janny Dinh, Sreya Sarkar, Garth J. Simpson, "Development of a temporal multiplexed 3D beam-scanning Lissajous trajectory microscope for rapid multimodal volumetric imaging," Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971312 (9 March 2016);