15 March 2016 A linear algorithm for quantitative measure of corneal collagen fiber orientation using second harmonic generation microscopy
Author Affiliations +
Abstract
There is significant interest in the scientific community to develop a reliable and precise quantification of the direction of collagen fibers within the cornea in order to learn more about how collagen contributes to the cornea's shape and structure. Previous work has shown that quantification of these fibers' orientations is possible using Second Harmonic Generation (SHG) microscopy, a modality that utilizes the non-centrosymmetric properties of collagen to obtain details of their macromolecular structure. Many attempts at using SHG to this purpose result in whole volume approximations which do not take into account variations through the depth of the cornea. Additionally, other algorithms have used non-linear processes which limit computation time and result in sensitivity to sample area, resolution, and illumination. We propose a linear method for quantification of collagen fiber orientation which utilizes the precise sectioning properties of SHG and is independent of non-linear artifacts to aid in further classification of cornea structure.
Conference Presentation
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
James McLean, James McLean, Charles DiMarzio, Charles DiMarzio, } "A linear algorithm for quantitative measure of corneal collagen fiber orientation using second harmonic generation microscopy", Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971317 (15 March 2016); doi: 10.1117/12.2235289; https://doi.org/10.1117/12.2235289
PROCEEDINGS
7 PAGES + PRESENTATION

SHARE
Back to Top