12 April 2016 Super resolution imaging of HER2 gene amplification
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Abstract
HER2 positive breast cancer is currently examined by counting HER2 genes using fluorescence in situ hybridization (FISH)-stained breast carcinoma samples. In this research, two-dimensional super resolution fluorescence microscopy based on stochastic optical reconstruction microscopy (STORM), with a spatial resolution of approximately 20 nm in the lateral direction, was used to more precisely distinguish and count HER2 genes in a FISH-stained tissue section. Furthermore, by introducing double-helix point spread function (DH-PSF), an optical phase modulation technique, to super resolution microscopy, three-dimensional images were obtained of HER2 in a breast carcinoma sample approximately 4 μm thick.
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Masaya Okada, Masaya Okada, Takuya Kubo, Takuya Kubo, Kanako Masumoto, Kanako Masumoto, Shigeki Iwanaga, Shigeki Iwanaga, } "Super resolution imaging of HER2 gene amplification", Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140E (12 April 2016); doi: 10.1117/12.2213918; https://doi.org/10.1117/12.2213918
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