Multi-pulse pumping for far-field super-resolution imaging

Sebastian Requena; Sangram Raut; Hung Doan; Joe Kimball; Rafal Fudala; Julian Borejdo; Ignacy Gryczynski; Yuri Strzhemechny; Zygmunt Gryczynski

doi:10.1117/12.2221921

1 March 2016 Multi-pulse pumping for far-field super-resolution imaging

Sebastian Requena, Sangram Raut, Hung Doan, Joe Kimball, Rafal Fudala, Julian Borejdo, Ignacy Gryczynski, Yuri Strzhemechny, Zygmunt Gryczynski

Author Affiliations +

Proceedings Volume 9714, Single Molecule Spectroscopy and Superresolution Imaging IX; 97140F (2016) https://doi.org/10.1117/12.2221921
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Recently, far-field optical imaging with a resolution significantly beyond diffraction limit has attracted tremendous attention allowing for high resolution imaging in living objects. Various methods have been proposed that are divided in to two basic approaches; deterministic super-resolution like STED or RESOLFT and stochastic super-resolution like PALM or STORM. We propose to achieve super-resolution in far-field fluorescence imaging by the use of controllable (on-demand) bursts of pulses that can change the fluorescence signal of long-lived component over one order of magnitude. We demonstrate that two beads, one labeled with a long-lived dye and another with a short-lived dye, separated by a distance lower than 100 nm can be easily resolved in a single experiment. The proposed method can be used to separate two biological structures in a cell by targeting them with two antibodies labeled with long-lived and short-lived fluorophores.

Citation Download Citation

Sebastian Requena, Sangram Raut, Hung Doan, Joe Kimball, Rafal Fudala, Julian Borejdo, Ignacy Gryczynski, Yuri Strzhemechny, and Zygmunt Gryczynski "Multi-pulse pumping for far-field super-resolution imaging", Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140F (1 March 2016); https://doi.org/10.1117/12.2221921

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KEYWORDS

Nanoparticles

Super resolution

Luminescence

Microscopy

Image resolution

Microscopes

Confocal microscopy

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