1 March 2016 Multi-pulse pumping for far-field super-resolution imaging
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Abstract
Recently, far-field optical imaging with a resolution significantly beyond diffraction limit has attracted tremendous attention allowing for high resolution imaging in living objects. Various methods have been proposed that are divided in to two basic approaches; deterministic super-resolution like STED or RESOLFT and stochastic super-resolution like PALM or STORM. We propose to achieve super-resolution in far-field fluorescence imaging by the use of controllable (on-demand) bursts of pulses that can change the fluorescence signal of long-lived component over one order of magnitude. We demonstrate that two beads, one labeled with a long-lived dye and another with a short-lived dye, separated by a distance lower than 100 nm can be easily resolved in a single experiment. The proposed method can be used to separate two biological structures in a cell by targeting them with two antibodies labeled with long-lived and short-lived fluorophores.
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Sebastian Requena, Sebastian Requena, Sangram Raut, Sangram Raut, Hung Doan, Hung Doan, Joe Kimball, Joe Kimball, Rafal Fudala, Rafal Fudala, Julian Borejdo, Julian Borejdo, Ignacy Gryczynski, Ignacy Gryczynski, Yuri Strzhemechny, Yuri Strzhemechny, Zygmunt Gryczynski, Zygmunt Gryczynski, } "Multi-pulse pumping for far-field super-resolution imaging", Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140F (1 March 2016); doi: 10.1117/12.2221921; https://doi.org/10.1117/12.2221921
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