1 March 2016 Correlating structure and fluorescence dynamics of quantum dot clusters using super-resolution imaging
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Abstract
Clusters of quantum dots exhibit fluorescent behavior that differs from that of individual particles. Bulk measurements involving a large number of particles obscure these dynamics. Synthesizing clusters with 5–10 particles enables the study of collective behavior where single-molecule fluorescence techniques can be applied. Super-resolution microscopy of these clusters correlated with SEM imaging reveals the influence of geometry and structure on emission dynamics. Signatures of energy transfer can be seen in the form of enhanced blinking. Motion of the emission center of the cluster is tracked, made possible by the independent blinking events of the individual particles. Discrete steps in the localization are observed as random switching between various on/off configurations moves the location of the emission center.
Conference Presentation
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Duncan P. Ryan, Duncan P. Ryan, Peter M. Goodwin, Peter M. Goodwin, Chris J. Sheehan, Chris J. Sheehan, Kevin J. Whitcomb, Kevin J. Whitcomb, Martin P. Gelfand, Martin P. Gelfand, Alan Van Orden, Alan Van Orden, } "Correlating structure and fluorescence dynamics of quantum dot clusters using super-resolution imaging", Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140T (1 March 2016); doi: 10.1117/12.2211150; https://doi.org/10.1117/12.2211150
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