1 March 2016 Novel plasmonic platform for ultra-sensitive detection and diagnostics
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Abstract
In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.
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Sangram L. Raut, Sangram L. Raut, Ryan Rich, Ryan Rich, Tanya Shtoyko, Tanya Shtoyko, Sebastian Requena, Sebastian Requena, Ilkay Bora, Ilkay Bora, Bo W. Laursen, Bo W. Laursen, Thomas Just Sørensen, Thomas Just Sørensen, Julian Borejdo, Julian Borejdo, Zygmunt Gryczynski, Zygmunt Gryczynski, Ignacy Gryczynski, Ignacy Gryczynski, } "Novel plasmonic platform for ultra-sensitive detection and diagnostics", Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 971419 (1 March 2016); doi: 10.1117/12.2221923; https://doi.org/10.1117/12.2221923
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