4 March 2016 All-in-one detector of circulating mRNA based on a smartphone
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Abstract
Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.
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Vratislav Cmiel, Vratislav Cmiel, Jaromir Gumulec, Jaromir Gumulec, Ondrej Svoboda, Ondrej Svoboda, Martina Raudenska, Martina Raudenska, Kristyna Hudcova, Kristyna Hudcova, Jiri Sekora, Jiri Sekora, Jaroslav Balogh, Jaroslav Balogh, Michal Masarik, Michal Masarik, Ivo Provaznik, Ivo Provaznik, } "All-in-one detector of circulating mRNA based on a smartphone", Proc. SPIE 9715, Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics, 971509 (4 March 2016); doi: 10.1117/12.2213253; https://doi.org/10.1117/12.2213253
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