Live dynamic OCT imaging of cardiac structure and function in mouse embryos with 43 Hz direct volumetric data acquisition

Shang Wang; Manmohan Singh; Andrew L. Lopez III; Chen Wu; Raksha Raghunathan; Alexander Schill; Jiasong Li; Kirill V. Larin; Irina V. Larina

doi:10.1117/12.2210751

9 March 2016 Live dynamic OCT imaging of cardiac structure and function in mouse embryos with 43 Hz direct volumetric data acquisition

Shang Wang, Manmohan Singh, Andrew L. Lopez III, Chen Wu, Raksha Raghunathan, Alexander Schill, Jiasong Li, Kirill V. Larin, Irina V. Larina

Author Affiliations +

Proceedings Volume 9716, Optical Methods in Developmental Biology IV; 971603 (2016) https://doi.org/10.1117/12.2210751
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Efficient phenotyping of cardiac dynamics in live mouse embryos has significant implications on understanding of early mammalian heart development and congenital cardiac defects. Recent studies established optical coherence tomography (OCT) as a powerful tool for live embryonic heart imaging in various animal models. However, current four-dimensional (4D) OCT imaging of the beating embryonic heart largely relies on gated data acquisition or postacquisition synchronization, which brings errors when cardiac cycles lack perfect periodicity and is time consuming and computationally expensive. Here, we report direct 4D OCT imaging of the structure and function of cardiac dynamics in live mouse embryos achieved by employing a Fourier domain mode-locking swept laser source that enables ~1.5 MHz A-line rate. Through utilizing both forward and backward scans of a resonant mirror, we obtained a ~6.4 kHz frame rate, which allows for a direct volumetric data acquisition speed of ~43 Hz, around 20 times of the early-stage mouse embryonic heart rate. Our experiments were performed on mouse embryos at embryonic day 9.5. Time-resolved 3D cardiodynamics clearly shows the heart structure in motion. We present analysis of cardiac wall movement and its velocity from the primitive atrium and ventricle. Our results suggest that the combination of ultrahigh-speed OCT imaging with live embryo culture could be a useful embryonic heart phenotyping approach for mouse mutants modeling human congenital heart diseases.

Citation Download Citation

Shang Wang, Manmohan Singh, Andrew L. Lopez III, Chen Wu, Raksha Raghunathan, Alexander Schill, Jiasong Li, Kirill V. Larin, and Irina V. Larina "Live dynamic OCT imaging of cardiac structure and function in mouse embryos with 43 Hz direct volumetric data acquisition", Proc. SPIE 9716, Optical Methods in Developmental Biology IV, 971603 (9 March 2016); https://doi.org/10.1117/12.2210751

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Heart

Optical coherence tomography

Data acquisition

Ultrafast imaging

Imaging systems

3D image processing

Animal model studies

Show All Keywords

Keywords/Phrases

Search In:

Publication Years