Comparison of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study

Chen Wu; Shihao Ran; Henry H. Le; Manmohan Singh; Irina V. Larina; David Mayerich; Mary E. Dickinson; Kirill V. Larin

doi:10.1117/12.2209147

9 March 2016 Comparison of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study

Chen Wu, Shihao Ran, Henry H. Le, Manmohan Singh, Irina V. Larina, David Mayerich, Mary E. Dickinson, Kirill V. Larin

Author Affiliations +

Proceedings Volume 9716, Optical Methods in Developmental Biology IV; 97160B (2016) https://doi.org/10.1117/12.2209147
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The mouse is a common model for studying developmental diseases. Different optical techniques have been developed to investigate mouse embryos, but each has its own set of limitations and restrictions. In this study, we imaged the same E9.5 mouse embryo with rotational imaging Optical Coherence Tomography (RI-OCT) and Selective Plane Illumination Microscopy (SPIM), and compared the two techniques. Results demonstrate that both methods can provide images with micrometer-scale spatial resolution. The RI-OCT technique was developed to increase imaging depth of OCT by performing traditional OCT imaging at multiple sides and co-registering the images. In SPIM, optical sectioning is achieved by illuminating the sample with a sheet of light. In this study, the images acquired from both techniques are compared with each other to evaluate the benefits and drawbacks of each technique for embryonic imaging. Since 3D stacks can be obtained by SPIM from different angles by rotating the sample, it might be possible to build a hybrid setup of two imaging modalities to combine the advantages of each technique.

Citation Download Citation

Chen Wu, Shihao Ran, Henry H. Le, Manmohan Singh, Irina V. Larina, David Mayerich, Mary E. Dickinson, and Kirill V. Larin "Comparison of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study", Proc. SPIE 9716, Optical Methods in Developmental Biology IV, 97160B (9 March 2016); https://doi.org/10.1117/12.2209147

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