Watching embryonic development in a new light: elasticity specific imaging with dual Brillouin/Raman microspectroscopy

Zhaokai Meng; Jessica A. Hanson; Vladislav V. Yakovlev

doi:10.1117/12.2213978

2 May 2016 Watching embryonic development in a new light: elasticity specific imaging with dual Brillouin/Raman microspectroscopy

Zhaokai Meng, Jessica A. Hanson, Vladislav V. Yakovlev

Author Affiliations +

Proceedings Volume 9716, Optical Methods in Developmental Biology IV; 97160L (2016) https://doi.org/10.1117/12.2213978
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Mechanical properties of tissues play an important role in biological development. However, the current elasticity-specific imaging techniques are either destructive / invasive, or have a limited spatial and/or temporal resolution. Recently, we introduced Brillouin microscopy imaging as a local non-invasive probe of microscopic viscoelasticity in cells and tissues. In this study, by taking advantage of Brillouin spectroscopy, we imaged the viscoelasticity properties of different compartments of living zebrafish embryos, including yolk-sac, skin, spine and heart. Brillouin and Raman spectra were collected simultaneously at each location using a recently developed Brillouin/Raman microscope.

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Citation Download Citation

Zhaokai Meng, Jessica A. Hanson, and Vladislav V. Yakovlev "Watching embryonic development in a new light: elasticity specific imaging with dual Brillouin/Raman microspectroscopy", Proc. SPIE 9716, Optical Methods in Developmental Biology IV, 97160L (2 May 2016); https://doi.org/10.1117/12.2213978

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