Light-sheet optimization for microscopy

Dean Wilding; Paolo Pozzi; Oleg Soloviev; Gleb Vdovin; Michel Verhaegen

doi:10.1117/12.2210912

15 March 2016 Light-sheet optimization for microscopy

Dean Wilding, Paolo Pozzi, Oleg Soloviev, Gleb Vdovin, Michel Verhaegen

Proceedings Volume 9717, Adaptive Optics and Wavefront Control for Biological Systems II; 971718 (2016) https://doi.org/10.1117/12.2210912
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM) configuration modified with the addition of a spatial light modulator (SLM) and a third objective for the detection of transmitted light. Optimization protocols use this transmission light allowing the extension the depth-of-field and correction of aberrations whilst retaining a thin optical section.

Conference Presentation

Citation Download Citation

Dean Wilding, Paolo Pozzi, Oleg Soloviev, Gleb Vdovin, and Michel Verhaegen "Light-sheet optimization for microscopy", Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 971718 (15 March 2016); https://doi.org/10.1117/12.2210912

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