15 March 2016 Light-sheet optimization for microscopy
Author Affiliations +
Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM) configuration modified with the addition of a spatial light modulator (SLM) and a third objective for the detection of transmitted light. Optimization protocols use this transmission light allowing the extension the depth-of-field and correction of aberrations whilst retaining a thin optical section.
Conference Presentation
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Dean Wilding, Dean Wilding, Paolo Pozzi, Paolo Pozzi, Oleg Soloviev, Oleg Soloviev, Gleb Vdovin, Gleb Vdovin, Michel Verhaegen, Michel Verhaegen, } "Light-sheet optimization for microscopy", Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 971718 (15 March 2016); doi: 10.1117/12.2210912; https://doi.org/10.1117/12.2210912

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