Prospects and challenges of quantitative phase imaging in tumor cell biology

Björn Kemper; Martin Götte; Burkhard Greve; Steffi Ketelhut

doi:10.1117/12.2213488

9 March 2016 Prospects and challenges of quantitative phase imaging in tumor cell biology

Björn Kemper, Martin Götte, Burkhard Greve, Steffi Ketelhut

Proceedings Volume 9718, Quantitative Phase Imaging II; 97180N (2016) https://doi.org/10.1117/12.2213488
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Quantitative phase imaging (QPI) techniques provide high resolution label-free quantitative live cell imaging. Here, prospects and challenges of QPI in tumor cell biology are presented, using the example of digital holographic microscopy (DHM). It is shown that the evaluation of quantitative DHM phase images allows the retrieval of different parameter sets for quantification of cellular motion changes in migration and motility assays that are caused by genetic modifications. Furthermore, we demonstrate simultaneously label-free imaging of cell growth and morphology properties.

Citation Download Citation

Björn Kemper, Martin Götte, Burkhard Greve, and Steffi Ketelhut "Prospects and challenges of quantitative phase imaging in tumor cell biology", Proc. SPIE 9718, Quantitative Phase Imaging II, 97180N (9 March 2016); https://doi.org/10.1117/12.2213488

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