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27 April 2016 Phase correlation imaging (PCI) for cell dynamics investigation (Conference Presentation)
Lihong Ma, Rajshekhar Gannavarpu, Ru Wang, Basanta Bhaduri, Shamira Sridharan, Mustafa A. Mir, Arindam Chakraborty, Supriya G. Prasanth, Larry Millet, Martha U. Gillette, Gabriel Popescu
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Proceedings Volume 9718, Quantitative Phase Imaging II; 97180P (2016) https://doi.org/10.1117/12.2217666
Event: SPIE BiOS, 2016, San Francisco, California, United States
Abstract
We present a new method, referred to as phase correlation imaging (PCI), to study cell dynamics and function through temporal phase correlation analysis. PCI offers label-free, high-performance, simple-design, as well as suitability for operation in a conventional microscopy setting. PCI works without the need for controlled or synchronized photoactivation and sophisticated acquisition schemes, and only involves taking a sequence of phase images. The PCI image incorporates information on the phase fluctuations induced by both Brownian motion and deterministic motion of intracellular transport across large scales. We employed spatial light interference microscopy (SLIM) recently developed in our laboratory to experimentally measure quantitative phase information which renders the thickness and refractive index of cellular components without adding contrast agents. The acquisition process is repeated to obtain time-lapse phase images. We calculate the correlation time at each pixel for acquired time-lapse phase images and obtain the correlation time map in space. By temporal correlation analysis, PCI reveals cell dynamics information, which is complementary to quantitative phase imaging itself.
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View presentation recording on the SPIE Digital Library: http://dx.doi.org/10.1117/12.2217666.4848767818001
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Lihong Ma, Rajshekhar Gannavarpu, Ru Wang, Basanta Bhaduri, Shamira Sridharan, Mustafa A. Mir, Arindam Chakraborty, Supriya G. Prasanth, Larry Millet, Martha U. Gillette, and Gabriel Popescu "Phase correlation imaging (PCI) for cell dynamics investigation (Conference Presentation)", Proc. SPIE 9718, Quantitative Phase Imaging II, 97180P (27 April 2016); https://doi.org/10.1117/12.2217666
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KEYWORDS
Microscopy
Phase measurement
Phase imaging
Image processing
Refractive index
Current controlled current source
Ruthenium
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