Characterizations of individual human red blood cells from patients with diabetes mellitus
(Conference Presentation)

SangYun Lee; Seongsoo Jang; HyunJoo Park; YongKeun Park

doi:10.1117/12.2214861

27 April 2016 Characterizations of individual human red blood cells from patients with diabetes mellitus (Conference Presentation)

SangYun Lee, Seongsoo Jang, HyunJoo Park, YongKeun Park

Author Affiliations +

Proceedings Volume 9718, Quantitative Phase Imaging II; 97180X (2016) https://doi.org/10.1117/12.2214861
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We systematically measure the morphological, biochemical, and biomechanical properties of individual human red blood cells (RBCs) from patients with diabetes mellitus using quantitative phase imaging technique to characterize the diabetic red cells with respect to those of the healthy. The 3-D refractive index tomograms and 2-D dynamic membrane fluctuation maps of individual RBCs are reconstructed from a set of the retrieved complex optical fields at various laser incidence angles using the Common-path diffraction optical tomography, from which volume, surface area, sphericity, hemoglobin (Hb) concentration, Hb content, and membrane fluctuation are obtained simultaneously. The correlative relations among the retrieved red cell indices of diabetic and healthy RBCs are also investigated with capabilities of individual cell measurement. As expected, there are no significant alterations in morphologies (cellular volumes, surface area, and sphericity) between diabetic and healthy RBCs. However, despite the minute mean corpuscular Hb differences in cell blood count datasheet, the measured Hb concentrations and Hb contents of diabetic RBCs are statistically higher than those of healthy RBCs, which might be related to the glycation of Hb molecules by hyperglycemia. Meanwhile, the membrane fluctuations of diabetic RBCs are clearly diminished compared to healthy red cells, implying the significantly decreased RBC deformability. In particular, it seems that the membrane fluctuations have mild negative relationships with the reported HbA1c levels.

Conference Presentation

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SangYun Lee, Seongsoo Jang, HyunJoo Park, and YongKeun Park "Characterizations of individual human red blood cells from patients with diabetes mellitus (Conference Presentation)", Proc. SPIE 9718, Quantitative Phase Imaging II, 97180X (27 April 2016); https://doi.org/10.1117/12.2214861

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KEYWORDS

Blood

Phase imaging

Diffraction

Molecules

Optical tomography

Refractive index

Current controlled current source

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