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27 April 2016 Studying the relationship between redox and cell growth using quantitative phase imaging (Conference Presentation)
Shamira Sridharan, Matthew T. Leslie, Natalya Bapst, John Smith, H. Rex Gaskins, Gabriel Popescu
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Proceedings Volume 9718, Quantitative Phase Imaging II; 971829 (2016) https://doi.org/10.1117/12.2216265
Event: SPIE BiOS, 2016, San Francisco, California, United States
Abstract
Quantitative phase imaging has been used in the past to study the dry mass of cells and study cell growth under various treatment conditions. However, the relationship between cellular redox and growth rates has not yet been studied in this context. This study employed the recombinant Glrx-roGFP2 redox biosensor targeted to the mitochondrial matrix or cytosolic compartments of A549 lung epithelial carcinoma cells. The Glrx-roGFP2s biosensor consists of a modified GFP protein containing internal cysteine residues sensitive to the local redox environment. The formation/dissolution of sulfide bridges contorts the internal chromophore, dictating corresponding changes in florescence emission that provide direct measures of the local redox potential. Combining 2-channel florescent imaging of the redox sensor with quantitative phase imaging allowed observation of redox homeostasis alongside measurements of cellular mass during full cycles of cellular division. The results indicate that mitochondrial redox showed a stronger inverse correlation with cell growth than cytoplasmic redox states; although redox changes are restricted to a 5% range. We are now studying the relationship between mitochondrial redox and cell growth in an isogenic series of breast cell lines built upon the MCF-10A genetic background that vary both in malignancy and metastatic potential.
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View presentation recording on the SPIE Digital Library: http://dx.doi.org/10.1117/12.2216265.4848767842001
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Shamira Sridharan, Matthew T. Leslie, Natalya Bapst, John Smith, H. Rex Gaskins, and Gabriel Popescu "Studying the relationship between redox and cell growth using quantitative phase imaging (Conference Presentation)", Proc. SPIE 9718, Quantitative Phase Imaging II, 971829 (27 April 2016); https://doi.org/10.1117/12.2216265
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KEYWORDS
Phase imaging
Biosensors
Breast
Bridges
Chromophores
Environmental sensing
Green fluorescent protein
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