9 March 2016 3D measurements of live cells via digital holographic microscopy and terahertz spectroscopy
Author Affiliations +
Proceedings Volume 9718, Quantitative Phase Imaging II; 97182D (2016) https://doi.org/10.1117/12.2213765
Event: SPIE BiOS, 2016, San Francisco, California, United States
Abstract
This is a study of the central nervous system (CNS) cells, including brain micro vascular endothelial cells (BMV) that constitute the blood brain barrier, and C6 glial cells that are the predominant cell in the brain. The cells are exposed to various chemicals by non-invasive, label-free methods. Digital holographic microscopy (DHM) is a technique that records an interference pattern between an object and reference waves, so that the computationally reconstructed holographic image contains both amplitude and phase information, and 3D images are obtained. The measurement of cell cultures by digital holographic microscopy yields information about cell death mechanisms, since these processes are correlated with individual cell volume. Our in-house DHM combines a visible (red) laser source with a conventional microscope base, and LabVIEW-run data processing. Terahertz spectral signatures are associated with structural changes in molecules and provide complementary information about cells. Both CNS cells BMV and C6 cells are treated with the drug “Methamphetamine" (METH), which induces apoptosis in neuronal cells and exhibits decrease in cell volume, a characteristic of cells undergoing apoptosis (induced cell death). METH can cause CNS cell death by cross-talk between mitochondria-, endoplasmic reticulum-, and receptor-mediated apoptotic events, all of which results in drug induced changes in neuroplasticity and significant neuropathology. Doxorubicin (DOX), a popular anticancer drug, is used as a control. We observe that METH treatment resulted in more pronounced cell volume shrinkage in both the BMV and C6 cells, as compared to DOX-induced cell apoptosis.
Conference Presentation
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jun Yong Park, Jun Yong Park, Dorian Oser, Dorian Oser, Peter Iapozzuto, Peter Iapozzuto, Sean Norbury, Sean Norbury, Supriya Mahajan, Supriya Mahajan, Alexander Khmaladze, Alexander Khmaladze, Anna Sharikova, Anna Sharikova, "3D measurements of live cells via digital holographic microscopy and terahertz spectroscopy", Proc. SPIE 9718, Quantitative Phase Imaging II, 97182D (9 March 2016); doi: 10.1117/12.2213765; https://doi.org/10.1117/12.2213765
PROCEEDINGS
6 PAGES + PRESENTATION

SHARE
Back to Top