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9 March 2016 Differentiating quiescent cancer cell populations in heterogeneous samples with fluorescence lifetime imaging
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Abstract
Measurement of relative fluorescence intensities of NAD(P)H and FAD with fluorescence lifetime imaging (FLIM) allows metabolic characterization of cancerous populations and correlation to treatment response. However, quiescent populations of cancer cells introduce heterogeneity to the tumor and exhibit resistance to standard therapies, requiring a better understanding of this influence on treatment outcome. Significant differences were observed between proliferating and quiescent cell populations upon comparison of respective redox ratios (p<0.05) and FAD lifetimes (p<0.05) across monolayers and in mixed samples. These results demonstrate that metabolic activity may function as a marker for separation and characterization of proliferating and quiescent cancer cells within mixed samples, contributing to comprehensive investigation of heterogeneity-dependent drug resistance.
Conference Presentation
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Tiffany M. Heaster, Alex J. Walsh, and Melissa C. Skala "Differentiating quiescent cancer cell populations in heterogeneous samples with fluorescence lifetime imaging", Proc. SPIE 9719, Biophysics, Biology, and Biophotonics: the Crossroads, 97190L (9 March 2016); https://doi.org/10.1117/12.2211734
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